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Biological sciences

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Subcuticular bacteria from the brittle star Ophiactis balli (Echinodermata: Ophiuroidea) represent a new lineage of extracellular marine symbionts in the alpha subdivision of the class Proteobacteria

Article Abstract:

The 16S ribosomal RNA (rRNA) gene sequence of the subcuticular bacterial symbionts (SCB) of Ophiactis balli was amplified bu eubacterium-specific polymerase chain reaction (PCR) primers. The resulting PCR product indicated the homology of the SCB gene sequence with the alpha subdivision of the class Proteobacteria. The SCB also represent a novel lineage of Proteobacteria that are not related to other known groups of bacterial symbionts from marine invertebrates.

Analysis, Ophiuroidea, Brittle stars, Bacteriology

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Genetic diversity among Xanthomonas campestris strains pathogenic for small grains

Article Abstract:

Indirect immunofluorescence pathogenicity tests and restriction fragment length polymorphism (RFLP) analysis help examine the genetic diversity among Xanthomonas campestris strains that are pathogenic for small grains. Pathogenicity tests on barley and serological tests reveal similarity among few strains, while they exhibit heterogeneity by RFLP analysis. Pathogenicity tests should be carried out before naming the strains at the pathovar level.

Bacteria, Pathogenic, Pathogenic bacteria

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Construction and characterization of a DNA probe for distinguishing strains of Aspergillus flavus

Article Abstract:

DNA fingerprinting techniques help design a molecular hybridization probe, which helps differentiate strains of Aspergillus flavus belonging to different vegetative compatibility groups. The DNA probe has a chromosomal DNA insert of A. flavus var.flavus NRRL 6541. Hybridization of the DNA probe to various DNA fragments of the parent A. flavus NRRL 6541 reveals the presence of repetitive DNA sequence in the cloned probe.

Aspergillus, DNA probes

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