Substrate selectivity and biochemical properties of 4-hydroxy- 2-keto-pentanoic acid aldolase from Escherichia coli
Article Abstract:
A study was conducted to determine 4-hydroxy-2-keto-pentanoic acid aldolase from Escherichia coli as a class I aldolase. The substrate selectivity for the reverse reaction was examined against the carbonyl donor pyruvic acid and the carbonyl acceptor acetaldehyde. Results indicated that the enzyme supported a high selectivity for the acetaldehyde acceptor. It also accepted alpha-ketobutyric acid or phenylpyruvic acid in place of the pyruvic acid carbonyl donor.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Relationship between membrane damage and cell death in pressure-treated Escherichia coli cells: differences between exponential- and stationary-phase cells and variation among strains
Article Abstract:
Research has been conducted on the membrane damage in Escherichia coli strains. The relationship between membrane damage and loss of viability following the pressure treatment has been investigated.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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Characterization of a bifunctional enzyme fusion of trehalose-6-phosphate synthetase and trehalose-6-phosphate phosphatase of Escherichia coli
Article Abstract:
Research has been conducted on the enzymes that catalyze sequential reactions. The effect of the physical proximity of these enzymes has been tested.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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