The RNA-DNA hybrid maintains the register of transcription by preventing backtracking of RNA polymerase

Article Abstract:

Polymerase chain reaction was performed to generate copies of DNA used for the RNA-DNA cross-links that were analysed to determine their functional role in ternary elongation complex. It was demonstrated that an 8- to 9-bp hybrid in the TEC is required to keep the RNA 3' terminus engaged with the active site of E. coli RNA polymerase. The results also showed that when hybrids are destabilized, detachment of the transcript terminus occurs and the RNA polymerase backtracks while the hybrid shifts upstream.

Nucleic acid hybridization, Polymerase chain reaction, Genetic transcription, Transcription (Genetics)

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Crystal structure of Thermus aquaticus core RNA polymerase at 3.3-angstrom resolution

Article Abstract:

Thermus aquaticus core RNA polymerase crystal structure is discussed as seen from X-ray crystallography at 3.3-angstrom resolution. A crab claw shape can be seen and has a 27-angstrom-wide internal channel. On the back wall of the channel is a Mg(super.2+) ion that is necessary for catalytic activity. It is chelated by an absolutely conserved motif of all eukaryotic and bacterial cellular RNA polymerases. Key functional sites are on the inner walls of the channel close to the active center.

Crystals, Crystal structure, X-ray crystallography

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Structural mechanism for rifampicin inhibition of bacterial RNA polymerase

Article Abstract:

The crystal structure of Thermus aquaticus core RNA polymerase (RNAP) complexed with rifampicin (Rif) has been determined. The structure, together with biochemical results, gives an explanation for the effects of Rif on RNAP function and indicates that the inhibitor acts by directly blocking the path of the elongating RNA when the transcript becomes 2-3 nt long.

Research, Physiological aspects, Tuberculosis, Cytochemistry, Antitubercular agents, Rifampin, Drug receptors

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