The antimicrobial effect of a structural variant of subtilin against outgrowing Bacillus cereus T spores and vegetative cells occurs by different mechanisms
Article Abstract:
The unusual dehydroalanine resdue at position 5 transformed into alanine in the construction of a structural variant of subtilin by site directed mutagenesis. The posttranslational processing of the precursor peptide was not disrupted by the mutation by subjecting the bacterial strains to proton nuclear magnetic resonance spectroscopy, amino acid composition analysis and N-terminal sequenceanalysis. It was shown that differences occur between the molecular mechanism that inhibits spore outgrowth and that which inhibits vegetative cells.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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Biosynthesis of the lantibiotic subtilin is regulated by a histidine kinase/response regulator system
Article Abstract:
The regulation of subtilin biosynthesis is discussed. Two genes are involved in this process, spaR and spaK. These strains were subjected to plasmid isolation, Southern hybridization, DNA sequencing, polymerase chain reaction and subtilin bioassay.Subtilin-negative mutants were derived with the deletion of any of the genes. Results indicate that subtilin biosynthesis are regulated by the two genes.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
User Contributions:
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