The chlorobenzoate dioxygenase genes of Burkholderia sp. strain NK8 involved in the catabolism of chlorobenzoates
Article Abstract:
The chlorobenzoate dioxygenase genes of Burkholderia sp. strain NK8, which grows well on 3-chlorobenzoate, 4-chlorobenzoate, and benzoate, have been found to be involved in the catabolism of chlorobenzoates. Chlorobenzoates are the major intermediate products of aerobic catabolism of polychlorinated biphenyls (PCBs). To examine the genes of chlorobenzoate dioxygenase or dioxygenases responsible for the broad substrate specificity of NK8 for chlorobenzoates a study was carried out. The chlorobenzoate dioxygenase genes of NK8 were cloned and analyzed using genetic and enzyme methods.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 2001
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Recombination of a 3-chlorobenzoate catabolic plasmid from Alcaligenes eutrophus NH9 mediated by direct repeat elements
Article Abstract:
The plasmid pENH91 from Alcaligenes eutophus NH9 that codes the proteins which catabolize 3-chlorobenzoate (3-CB) is characterized. The catabolite genes are located in a 12.5 kb region of the plasmid that is formed by the reciprocal recombination of two identical sequences. Subculturing of NH9 on liquid media with 3-CB yields a strain, NH9A, which possesses another copy of the catabolite genes probably formed by recombination of the repeat sequences.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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The chlorocatechol-catabolic transposon Tn5707 of Alcaligenes eutrophus NH9, carrying a gene cluster highly homologous to that in the 1, 2, 4-trichlorobenzene-degrading bacterium Pseudomonas sp. strain P51, confers the ability to grow on 3-chlorobenzoate
Article Abstract:
Research was conducted to examine the structure of genes for chlorocatechol-degrading enzymes of Alcaligenes eutrophus NH9 which was isolated in Japan. The gene utilizes 3-chlorobenzoate as its sole source of carbon and energy. Sequencing of the relevant region of plasmid pENH91 from strain NH9 showed that the genes for the catabolic enzymes were homologous to the genes of the modified ortho-cleavage pathway. Results indicate the prevalence of identical insertion sequences among bacteria.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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