The diacetamidodideoxyuronic-acid-containing glycan chain of Bacillus stearothermophilus NRS 2004/3a represents the secondary cell-wall polymer of wild-type B. stearothermophilus strains
Article Abstract:
Research was conducted to define the linkage type of the ManpA,2,3(NAc)2-containing glycan to the cell wall of Bacillus stearothermophilus NRS 2004/3a. The affinity between the S-layer glycoprotein and the coeluting ManpA2,3(NAc)2-containing glycan was also investigated. Results reveal that there is only low, if any, specific affinity of the secondary cell-wall polymer to the intact S-layer protein, an observation that is supported by the previous separation experiments of proteolytically degraded S-layer glycoprotein preparations that gave rise to a complete splitting of both carbohydrate structures.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1999
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Isolation and characterization of the gene encoding single-stranded-DNA-binding protein (SSB) from four marine Shewanella strains that differ in their temperature and pressure optima for growth
Article Abstract:
The cloning and sequencing of the single-stranded-DNA-binding-protein (SSB) genes from four Shewanella strains and the overproduction and partial purification of the SSB proteins were presented. The cloned strains varied in their temperature and pressure optima and ranges for growth. Differences observed among the Shewanella SSB suggest that these proteins will provide a useful system for exploring the adaptation of protein-protein and protein-DNA interactions at low temperature and high pressure.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
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Identification and analysis of a gene (abpA) encoding a major amylase-binding protein in Streptococcus gordonii
Article Abstract:
The gene abpA which codes for the 20 kiloDalton amylase-binding protein from the oral cavity colonizer Streptococcus gordonii was identified using a conjugative transposon that inactivates the genes necessary for the expression of this protein. Single-specific-primer polymerase chain reaction was used to sequence this gene which revealed an open reading frame 585 base pairs in length. The implications of these findings on the bacterial colonization of the oral cavity are discussed.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1998
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