The gene for gamma-glutamylcysteine synthetase from Thiobacillus ferroxidans has low homology to its Escherichia coli equivalent and is linked to the gene for citrate synthase

Article Abstract:

The gene for gamma-glutamylcysteine synthetase (gshA) from Thiobacillus ferrooxidans was isolated from a family of cosmids by its ability to complement an Escherichia coli gshA trxA double mutant which could not grow on minimal medium without glutathione. The predicted sequence of the enzyme had only 18% amino acid sequence similarity to the equivalent enzyme from E. coli. The gshA gene is physically and transcriptionally related to the T. ferroxidans gene for citrate synthase.

Ligases

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The pyruvate dehydrogenase complex of the chemolithoautotrophic bacterium Thiobacillus ferrooxidans has an unusual E2-E3 subunit fusion

Article Abstract:

The genes encoding pyruvate dehydrogenase (PDH) of Thiobacillus ferrooxidans were identified. The results showed that these genes complement an Escherichia coli aroP-Ipd mutant in growth on minimal medium lacking acetate. The observation indicates that the T. ferrooxidans PDH complex was functional in E. coli. Results reveal that T. ferrooxidans PDH complex have more features in common Gram-positive bacteria and eukaryotes, in spite of it being a Gram-negative bacterium.

Usage, Gram-negative bacteria

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The prpE gene of Salmonella typhimurium LT2 encodes propionyl-CoA synthetase

Article Abstract:

A study was conducted to show that the prpE gene of Salmonella typhimurium encodes propionyl-CoA synthetase, an enzyme needed for the catabolism of propionate in the bacterium. DNA manipulations were carried out in Escherichia coli DH5-alpha/F'. The plasmid pPRP38 was then utilized to overexpress prpE. Experimental results indicated that propionyl-CoA was the production of reactions catalyzed by PrpE.

Enzymes, Salmonella typhimurium

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