The global folding of four-way helical junction in RNA, including that in U1 snRNA

Article Abstract:

The four-way junctions in RNA molecules including U1 snRNA are formed by the pairwise coaxial stacking of the helical arms and there is a tendency for the molecules to change into a 90 degrees crossed structure. The molecules are present as two isomers which interconvert when the bases at the strand exchange site are changed. In the presence of Mg2+, the helical junctions are at 90 degrees while the RNA junctions show different conformations. The 90 degrees crossed conformation of the U1 snRNA molecules is unaffected by changes in bases or presence of ions.

Analysis, Molecular structure, Protein folding

---

DNA demethylation in vitro: involvement of RNA

Article Abstract:

The role of RNA in DNA demethylation in vitro was determined by demethylation assay using extracts from cultured myoblasts. The 5' phosphates of the C and G nucleotide residues were removed from the reaction center during the course of the reaction and were transferred to RNA molecules to generate the RNase-sensitive form. Treatment of extracts with proteinase K resulted in the loss of specificity of demethylation, suggesting that the reaction may be mediated by RNA molecules.

DNA, Methyltransferases

---

RNA structure comes of age

Article Abstract:

A meeting devoted to RNA structure was held at the University of California, Santa Cruz campus in June. The main theme was the huge progress made in determining structures of small modular units of RNA structure, and 30 such structures were presented. Several talks concentrated on the specific role of monovalent and divalent cations in RNA folding, stability and catalysis.

Conferences, meetings and seminars, Biochemistry

Similar abstracts:

• Abstracts: Inactivation of fecal bacteria in drinking water by solar heating. Novel cassette-based shuttle vector system for gram-positive bacteria
• Abstracts: Decoding of cytosolic calcium oscillations in the mitochondria. Altered Ca2+ responses in muscles with combined mitochondrial and cytosolic creatinine kinase deficiencies
• Abstracts: Microbial aldicarb transformation in aquifer, lake, and salt marsh sediments. Rapid anaerobic benzene oxidation with a variety of chelated Fe(III) forms
• Abstracts: The stabilization of repetitive tracts of DNA by variant repeats requires a functional DNA mismatch repair system
• Abstracts: Membrane topology analysis of the Escherichia coli cytosine permease. part 2 Isolation of Aspergillus niger creA mutants and effects of the mutations on expression of arabinases and L-arabinose catabolic enzymes

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.