The invariant U5 snRNA loop 1 sequence is dispensable for the first catalytic step of pre-mRNA splicing in yeast
Article Abstract:
An in vitro reconstitution system was developed to study the role of U5 snRNA in the two catalytic steps of pre-mRNA splicing. It is known that the invariant loop 1 sequence of U5 interacts with exon sequences at the 5' splice location before the first catalytic step. An in vitro analysis of U5 mutations shows that the first transesterification precisely occurs when the U5 loop is absent. Thus, this sequence is not a major component of the spliceosomal active site for the first catalytic step. The second catalytic step was found to tolerate mutations in the invariant sequence.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1996
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Mad cows meet psi-chotic yeast: the expansion of the prion hypothesis
Article Abstract:
Bovine spongiform encepalopathy is caused by mutations in the conformation of the cerebral PrP protein which generates an infectious material that induces neuronal cell death or apoptosis. Analysis of yeast prions such as [PSI+] and [URE3] indicated the relationship between phenotype inheritance and inheritance of different protein conformations. Furthermore, the genetic experiments with [PSI+] and [URE3] yeast elements demonstrated the presence of mechanism that induced heritable changes in protein conformation.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1997
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Invariant chain structure and MHC class II function
Article Abstract:
The mechanism employed by major histocompatibility complex (MHC) class II molecules to bind to peptides from the endosomal-lysosomal system is examined. The Ii chain which interacts with the MHC class II molecule has a type II membrane glycoprotein of 216 amino acid residues with an N-terminal cytoplasmic domain. Three class II alpha-beta dimers bind to the Ii chain trimer by interaction of their peptide-binding grooves with the CLIP region of the Ii chain.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1996
User Contributions:
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