The isolation of mutagen-sensitive nuv mutants of Aspergillus nidulans and their effects on mitotic recombination

Article Abstract:

Twenty-three mutants that were hypersensitive to N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and 4-nitroquinoline-1-oxide (4-NQO) were isolated and studied for their effects on spontaneous intragenic recombination. Ten of these had no significant effects on recombination. Reactions to different concentrations of MNNG and 4-NQO were varied. Most of the nuv mutations were fully recessive and only a few were semidominant. Six nonallelic loci were identified in trans complementation analysis of seven of these mutations.

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Effects of mutagen-sensitive mus mutations on spontaneous mitotic recombination in Aspergillus

Article Abstract:

The effects of eight mutations of the mus locus of Aspergillus nidulans on mitotic recombination were studied. Two of the mutations increased, while another two decreased inter- and intragenic recombination. The musO mutation increased only intragenic recombination. The product of the musL mutation appeared necessary for recombination and its absence resulted in chromosome loss. The behavior of some mutants suggested differential effects on the related events of mitotic gene conversion and crossing over.

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nuvA, an Aspergillus nidulans gene involved in DNA repair and recombination, is a homologue of Saccharomyces cerevisiae RAD18 and Neurospora crassa uvs-2

Article Abstract:

The nuv A gene plays an important role in DNA repair and recombination in Aspergillus nidulans. The cloning of the nuv A gene is undertaken by complementing it with the nuvA11 phenotype. The isolation of the nuv cDNA sequence is described and the location of the nuv A gene on chromosome IV is determined. Results indicate that the nuv A gene is a homologue of the known DNA repair proteins uvs-2 in Neurospora crassa and RAD18 in Saccharomyces cerevisiae.

DNA repair, Saccharomyces, Neurospora

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