The protoxin composition of Bacillus thuringiensis insecticidal inclusions affects solubility and toxicity

Article Abstract:

Incorporation of cloned copies of cryIAb gene into Bacillus thuringiensis induce changes in the composition of the CryIAb protoxin which results in increased solubility and decreased toxicity of the insecticidal inclusion proteins. A protoxin inclusion contains CryIAb, CryIC, and CryID protoxins. The effects on the solubility and toxicity of inclusions produced by the cured bacterium results from the loss of the cryIAb-carrying plasmid.

Physiological aspects, Plasmids, Bacterial toxins, Genetic regulation

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Characterization of two genes encoding Bacillus thuringiensis insecticidal crystal proteins toxic to coleoptera species

Article Abstract:

An investigation of the cause of the different insecticidal activities of two genes encoding Bacillus thuringiensis is discussed. Crystal protein III type genes toxic to coleopterans were cloned from each strain and their transcriptional start siteswere analysed. The two crystal proteins were found to have similar sequence. However, both gene products displayed distinct insecticidal activities.

Health aspects, Analysis, Beetles

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Binary toxins from Bacillus thuringiensis active against the western corn rootworm, Diabrotica virgifera virgifera LeConte

Article Abstract:

The two new classes of crystal proteins Bacillus thuringiensis strain EG4851 that comprise a binary toxin active against western corn rootworm (WCR) are described. The antirootworm proteins produced by strain EG4851 represent a new binary toxin and the genes encoding these proteins may become an important component of a sustainable resistance management strategy against this insect pest.

Science & research, Toxins

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