The tuf3 gene of Streptomyces coelicolor A3(2) encodes an inessential elongation factor Tu that is apparently subject to stringent control

Article Abstract:

A study conducted on EF-Tu3, a polypeptide chain elongation factor encoded by the tuf3 gene of Streptomyces coelicolor A3(2), shows that tuf3 is not necessary for growth as a mutation in tuf3 does not cause any growth defects. No tuf3 is formed in cells growing in liquid cultures. However cells stressed by a decrease in nutrients or the presence of serine hydroxamate, form tuf3. The tuf3 gene is also formed during the transition and stationary growth phases in the strain J1681 which does not contain the bldA gene. Transcription of tuf3 takes place only after the nucleotide ppGpp is formed.

Genetic regulation

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A response-regulator-like activator of antibiotic synthesis from Streptomyces coelicolor A3(2) with an amino-terminal domain that lacks a phosphorylation pocket

Article Abstract:

Streptomyces coelicolor A3(2) offers a good model system for the study of secondary metabolism, due to its well developed genetics and ability to produce different antibiotics. BldA mutants in Streptomyces coelicolor A3(2), lacking tRNA for the rare leucine codon UUA, cannot make the red undecylprodigiosin antibiotic complex. A cloning strategy was used to localize the red gene cluster, and a PWb mutation derivative of a bldA mutant, was located between red structural genes in the sector 1-4 kb from the pathway-specific regulatory gene redD.

Physiological aspects, Biosynthesis, Microbiological research

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Green fluorescent protein as a reporter for spatial and temporal gene expression in Streptomyces coelicolor A3(2)

Article Abstract:

Green fluorescent protein (GFP) is discussed relative to its function as a reporter for temporal and spatial gene expression in Streptomyces coelicolor A3(2). The enhanced GFP (EGFP) gene is a modified version of the GFP gene of a jellyfish with codon usage that corresponds closely to that found in many streptomycete genes that are rich in GC.

United Kingdom, Spain, Statistical Data Included, Analysis, Usage, Genetic aspects, Proteins, Gene expression, Cytochemistry, Chromosome mapping

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