The type 2C Ser/Thr phosphatase PP2C gamma is a pre-mRNA splicing factor
Article Abstract:
PP2C gamma, a type 2C Ser/Thr phosphatase, has been shown to be a pre-messenger-RNA splicing factor. Human PP2C gamma is associated physically with the spliceosome in vitro all the way through the splicing reaction. It is first needed in early stages of spliceosome assembly for efficient formation of the A complex; the phosphatase activity is necessary for the splicing action of PP2C gamma. One, at least, specific dephosphorlyation event catalyzed by PP2C gamma is necessary for the spliceosome's formation.
Publication Name: Genes & Development
Subject: Biological sciences
ISSN: 0890-9369
Year: 1999
User Contributions:
Comment about this article or add new information about this topic:
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins
Article Abstract:
Studies have uncovered new exonic splicing enhancer (ESE) classes recognized by human SR proteins. Immunoprecipitation and UV cross-linking/competition experiments demonstrated that SR proteins recognize the ESEs by specific and direct binding in nuclear extract. A wide variety of sequences can act as ESEs, and occur in exons at higher frequencies than in introns.
Publication Name: Genes & Development
Subject: Biological sciences
ISSN: 0890-9369
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
Pre-mRNA splicing in the absence of an SR protein RS domain
Article Abstract:
Research shows that the RS domain of SF2/ASF protein is not required for in vitro splicing of constitutive and enhancer-dependent pre-mRNAs. Data indicate that specific substrates and strength of splicing signals determine the requirement of this domain.
Publication Name: Genes & Development
Subject: Biological sciences
ISSN: 0890-9369
Year: 2000
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Phosphorylated RNA polymerase II stimulates pre-mRNA splicing. RNA polymerase II and the integration of nuclear events
- Abstracts: A bead-based method for multiplexed identification and quantitation of DNA sequences using flow cytometry. Stimulating the in situ activity of Geobacter species to remove uranium from the groundwater of a uranium-contminated aquifer
- Abstracts: Archaeal genomics: an overview. Aminoacyl-tRNAs: setting the limits of the genetic code. Aminoacyl-tRNA synthetases, the genetic code, and the evolutionary process
- Abstracts: Curing of F-like plasmid TP171 by plumbagin is due to interference with both replication and maintenance functions
- Abstracts: VDJ recombination: a transposase goes to work. Global role for chromatin remodeling enzymes in mitotic gene expression