The type 2C Ser/Thr phosphatase PP2C gamma is a pre-mRNA splicing factor

Article Abstract:

PP2C gamma, a type 2C Ser/Thr phosphatase, has been shown to be a pre-messenger-RNA splicing factor. Human PP2C gamma is associated physically with the spliceosome in vitro all the way through the splicing reaction. It is first needed in early stages of spliceosome assembly for efficient formation of the A complex; the phosphatase activity is necessary for the splicing action of PP2C gamma. One, at least, specific dephosphorlyation event catalyzed by PP2C gamma is necessary for the spliceosome's formation.

Genetic aspects, Recombinant DNA, Phosphatases, HeLa cells

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Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins

Article Abstract:

Studies have uncovered new exonic splicing enhancer (ESE) classes recognized by human SR proteins. Immunoprecipitation and UV cross-linking/competition experiments demonstrated that SR proteins recognize the ESEs by specific and direct binding in nuclear extract. A wide variety of sequences can act as ESEs, and occur in exons at higher frequencies than in introns.

Nucleotide sequence, Base sequence, Introns, Exon (Molecular genetics), Exons (Molecular genetics), Chromosomal proteins

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Pre-mRNA splicing in the absence of an SR protein RS domain

Article Abstract:

Research shows that the RS domain of SF2/ASF protein is not required for in vitro splicing of constitutive and enhancer-dependent pre-mRNAs. Data indicate that specific substrates and strength of splicing signals determine the requirement of this domain.

Analysis, Gene expression

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