The use of transposable P-elements of Drosophila melanogaster for introductory genetics laboratory courses

Article Abstract:

The insertion of the Drosophila melanogaster transposable P-elements in to the genome is a good method for gene transmission and mapping in introductory laboratory courses. The P-elements possessing inserts of wild type alleles of yellow, white and forked phenotype exhibit the Mendelian pattern of inheritance. The transposons can be inserted in various positions in the genome, stocks with good viability can be raised and inserts can be selected for mapping the maker genes with high precision. The inserts are mapped in the same locations in mapping experiments by different groups.

Author: Morcillo, P., Tuttle, R., Maclntyre, R.J.
Study and teaching, Chromosome mapping

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Permanent fixation of a transposable element insert in the A2 gene of maize (Zea mays L.)

Article Abstract:

Some genetic variations help organisms adapt to their environments. The presence of transposable elements can catalyze genetic change and is reflected in the case of footprint creation at positions where element excisions are made. The I element has been tested with the En/Spm to determine its stability in generating genetic change. It was shown that the element exhibited stability in both insertion and composition.

Author: Thatiparthi, V.R., Dinesh-Kumar, S.P., Peterson, P.A.
Genetic aspects, Corn, Gene expression, Insertion elements, DNA, DNA insertion elements, Genetic transformation

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Accumulation of transposable elements in the genome of Drosophila melanogaster is associated with a decrease in fitness

Article Abstract:

Presenting the data on transposable element (TE) copy numbers, fitness, and hatchability of replicates derived from two homozygous laboratory strains of Drosophila melanogaster-2b and Harwich. Assuming a weak deleterious effect of each TE insertion, a decrease in fitness is expected with an increase in genomic TE copy number.

Author: Pasyukova, E.G., Nuzhdin, S.V., Morozova, T.V., Mackay, T.F.C.
Science & research, Genetic research

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Subjects list: Research, Drosophila, Transposons
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