Thermostabilization of proteins by diglycerol phosphate, a new compatible solute from the hyperthermophile Archaeoglobus fulgidus

Article Abstract:

A new compatible solute from the hyperthermophile Archaeoglobus fulgidus is discussed. Thermostabilization of proteins by 1,1'- diglycerol phosphate (DGP) has been studied. It was found to be the major solute accumulating mainly in response to salt stress in the archaeon A. fulgidus. Chemical synthesis was reported on because the poor growth yields of A. fulgidus and large amounts of solute required made the organisms not a good source. Indirect evidence for the stereochemistry of the natural solute was found. Rubredoxins and their known structural features are related to this study. DGP is potentially useful as a protein stabilizer in biotech applications.

United States, Germany, Portugal, Thermophilic bacteria, Bacteria, Archaebacteria, Materials, Protein research, Biotechnology, Stereochemistry

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Organic solutes in hyperthermophilic Archaea

Article Abstract:

The major organic solutes that are accumulated by microbes at optimum growth temperature and salinity were analyzed in 12 species of thermophilic and hyperthermophilic Archaea. A novel phosphorylated solute identified as di-glycerol-phosphate was accumulated by Archaeoglobus fulgidus in response to salt and temperature stress. Furthermore, increasing levels of growth temperature led to the accumulation of di-myo-inositol-phosphate derivatives and cyclic-2,3-bisphosphoglycerate while salt stress led to the accumulation of glutamate isomers.

Environmental aspects, Microbial metabolism, Microbial growth, Thermophiles, Archaea

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Effects of temperature, salinity, and medium composition on compatible solute accumulation by Thermococcus spp

Article Abstract:

Nuclear magnetic resonance spectroscopy was used to investigate the effects of salinity and growth temperature on the accumulation of intracellular organic solutes in Thermococcus litoralis, T. celer, T. stetteri and T. zilligii. It was shown that T. celer, T. stetteri and T. litoralis accumulate different solutes in response to two different types of stress. The levels of aspartate, mannosylglycerate and beta-galactopyranosyl-5- hydroxyllysine increased in response to salt stress while the levels of di-myo-inositol-1,1'(3,3')-phosphate responded to temperature stress.

Nuclear magnetic resonance spectroscopy, Organic compounds, Salinity, Solution (Chemistry), Solutions (Chemistry), Temperature

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