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Biological sciences

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Transformation of Escherichia coli with DNA from Saccharomyces cerevisiae cell lysates

Article Abstract:

A system for monitoring transfer of heterologous DNA from a genetically altered strain of Saccharomyces cerevisiae to Escherichia coli has been developed. DNA transfer is measured by finding the number of ampicillin-resistant E. coli clones. The system is based on a yeast strain carrying integrated copies of a pUC-derived plasmid with bacterial sequences maintained in the yeast genome by selectable markers for lactose use. Results indicate that in ordinary circumstances spontaneous transfer of chromosomal genes from genetically modified organisms (GMOs) would be rare.

Spain, Statistical Data Included, Environmental aspects, Genetic aspects, Genetically modified animals, Genetically modified plants, Escherichia coli, Cytochemistry, Saccharomyces, Microbiological research, Biotechnological microorganisms, Bioengineering

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A novel strategy for control of microbial biofilms through generation of biocide at the biofilm-surface interface

Article Abstract:

Hydrogen peroxide (H2O2) and potassium monopersulfate (KMPS) produce active oxygen species that kill the Pseudomonas aeruginosa cells present on biofilms. The biocides produce oxygen species by breaking down peroxides and persulfates. The oxygen species act specifically on the bacteria on the biofilms. The addition of copper and cobalt phthalocyanine catalysts to the biofilms increases the antimicrobial activity of H2O2 and KMPS. The bacteria that survive after the treatment are loosely attached to the biofilms and are easily removed by normal cleaning methods.

Anti-infective agents, Active oxygen

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Electrical enhancement of biocide efficacy against Pseudomonas aeruginosa biofilms

Article Abstract:

The effects of a low-strength electrical field with a low current density (EF-CD) on the antimicrobial activity of commonly used biocides were investigated. The experiments were conducted on Pseudomonas aeruginosa biofilms, and the biocides tested were kathon, a quaternary ammonium compound and glutaraldehyde. The results showed that an EF-CD of +/-12 V/cm at +/-2.1 mA/squared centimeter could enhance the killing action of all three biocides.

Control, Pseudomonas aeruginosa, Electric fields

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