Transformation of Kluyveromyces lactis by electroporation
Article Abstract:
The optimum conditions for transforming Kluyveromyces lactis by electroporation were established. Using the Bio-Rad Gene Pulser and Pulse Controller, 10(super 7) transformants per microgram of DNA could be obtained by pretreatment with DTT and carefully selecting pulse time, which depended entirely on sample resistance. Maximum efficiency of transformation could also be assured by loading as many cells and as much plasmid DNA as possible in the cuvette.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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The KIPH05 gene encoding a repressible acid phosphatase in the yeast Kluyveromyces lactis: cloning, sequencing and transcriptional analysis of the gene, and purification and properties of the enzyme
Article Abstract:
Genetic research has identified a secreted phosphate-repressible acid phosphatase from the yeast organism Kluyveromyces lactis which contains the gene KIPH05. KIPH05 encodes the enzyme KIPho5p which has a molecular mass of 52520Da. It is not present in some strains of K. lactis according to Southern blot analysis. Northern blot research of total cellular RNA suggests that a 1.8 kb transript gene is encoded by KIPho5p.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
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Heterologous protein secretion directed by a repressible acid phosphatase system of Kluyveromyces lactis: characterization of upstream region-activating sequences in the KIPHO5 gene
Article Abstract:
The promoter region of the repressible acid phosphatase gene (KIPHO5) in Kluyveromyces lactis was examined using a combination of deletion and directed mutagenesis. A 456-bp upstream region with all the putative cis-acting regulatory elements was identified to determine important elements in the KIPHO5 promoter. Deletion mutants were constructed to screen the promoter region for functionally important DNA sequences.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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- Abstracts: Production of active chimeric pediocin AcH in Escherichia coli in the absence of processing and secretion genes from the Pediococcus pap operon
- Abstracts: Molecular cloning, sequencing, and expression of a chemoreceptor gene from Leptospirillum ferrooxidans. Copper ions stimulate polyphosphate degradation and phosphate efflux in Acidithiobacillus ferrooxidans