Transposon mutagenesis with IS6100 in the avermectin-producer Streptomyces avermitilis

Article Abstract:

Research was conducted to evaluate the intermolecular transposition of the insertion sequence IS6100 from the delivery plasmid to the genome of the avermectin-producer Streptomyces avermitilis. Results revealed the consistency of Southern hybridization with random transposition. Variances involving the frequency of reversion of different auxotrophic mutations by precise excision were also observed.

Mutagenesis

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Disruption of sblA in Streptomyces lividans permits expression of a heterologous alpha-amylase gene in the presence of glucose

Article Abstract:

Disruption of sblA in Streptomyces lividans is discussed. This disruption makes expression of a heterologous alpha-amylase gene in the presence of glucose possible. In a transposition mutant of S. lividans TK24, the usually glucose-repressible expression of a heterologous alpha amylase gene was rendered resistant to glucose repression.

France, Statistical Data Included, Usage, Physiological aspects, Genetic aspects, Cytochemistry, Chromosome mapping, Phosphatases, Glucose, Inositol

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