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Trehalose and sucrose protect both membranes and proteins in intact bacteria during drying

Article Abstract:

The sugars trehalose and sucrose may protect Escherichia (E.) coli DH5-alpha and Bacillus (B.) thuringiensis HD-1 from dying when they are freeze-dried. When the bacteria were freeze-dried in the presence of each sugar and then rehydrated, 44% to 70% of them survived. Without the sugars, only 8% of the E. coli and 14% of the B. thuringiensis survived. The protection is probably caused by the sugars' ability to lower the phase transition temperature of the bacterial membranes and protect protein structure.

Author: Crowe, John H., Crowe, Lois M., Israeli, Eitan, Lighthart, Bruce, Leslie, Samuel B.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
Observations, Bacteria, Proteins, Protein structure, Membranes (Biology), Sucrose

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Survival differences among freeze-dried genetically engineered with wild-type bacteria

Article Abstract:

The utilization of freeze-drying for evaluating the survival differences between two dry state genetically engineered and nonengineered bacteria is discussed. The effects of light, relative humidity and air were the parameters used on the engineered strains derived from Escherichia coli and Pseudomonas syringae. Better sensitivity to dry state air exposure was demonstrated by engineered Pseudomonas strains compared to their wild type parental strain.

Author: Israeli, Eitan, Shaffer, Brenda T., Hoyt, Julie A., Lighthart, Bruce, Ganio, Lisa M.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993

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Expression and immunogenicity of a recombinant diphtheria toxin fragment A in Streptococcus gordonii

Article Abstract:

The expression and immunogenicity of a nontoxic diphtheria toxin (DT) fragment A (DTA) in Streptococcus gordonii, with the long-term goal of developing a live oral diphtheria-pertussis-tetanus vaccine was investigated. A mutant DTA in single, double and triple copy was surface expressed in S. gordonii as fusion proteins to the SpaP protein.

Author: Lee, Song F., Lee, Chiang W., Halperin, Scott A.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
Science & research, Causes of, Streptococcus, Diphtheria toxin, Tetanus antitoxin, Tetanus toxoid

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Subjects list: Research, Usage, Freeze-drying, Freeze drying, Recombinant microbial toxins
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