Two-dimensional polyacrylamide gel electropherosis analysis of the acid tolerance response in Listeria monocytogenes LO28
Article Abstract:
The two-dimensional polycrylamide gel electrophoresis analysis of the acid tolerance response in Listeria monocytogenes LO20 was examined to describe alterations in protein levels on L. monocytogenes during acid habituation. The study used a two-dimensional SDS-polyacrylamide gel electrophoresis to examine protein alterations during acid adaptation. Results reveal alterations in protein synthesis during acid adaptation in L. monocytogenes that can lead to increased chances of survival under stressful conditions.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Rapid real-time PCR assay for detection and quantitation of Mycobacterium avium subsp. paratuberculosis DNA in artificially contaminated milk
Article Abstract:
A real-time PCR assay with primers and probes designed by using IS900, which allowed rapid detection of Mycobacterium avium subsp. paratuberculosis DNA in artificially contaminated milk was developed using fluorescence energy transfer technology and Lightcycler analysis. The entire procedure was performed in less than 3 hours and was successfully adapted to quantify M. avium subsp. paratuberculosis in spiked milk from heavily and mildly contaminated samples.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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Peptide aMptD-mediated capture PCR for detection of Mycobacterium avium subsp. paratuberculosis in bulk milk samples
Article Abstract:
A peptide-mediated capture PCR was developed and characterized for detection of Mycobacterium avium subsp. paratuberculosis in bulk milk samples using peptide aMptD. This method depends solely on synthetic reagents, does well under routine laboratory conditions and is adaptable to standard laboratory automation.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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