Two multifunctional peptide synthetases and an O-methyltransferase are involved in the biosynthesis of the DNA-binding antibiotic and antitumour agent saframycin Mx1 from Myxococcus xanthus
Article Abstract:
Examination of 14.1 kb DNA sequence involved in producing the antibiotic saframycin Mx1 from Myxococcus xanthus indicated two genes for two large multifunctional synthetases of 1770 and 2605 amino acids, respectively, and a putative O-methyltransferase of 220 amino acids. The peptide synthetases have two amino-acid-activating domains each. Mutant strains which do not produce the antibiotic because they lack one of the peptide synthetases secrete a substrate that can be employed by O-methyltransferase-deficient mutant strains to produce saframycin Mx1.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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A new Myxococcus xanthus gene cluster for the biosynthesis of the antibiotic saframycin Mx1 encoding a peptide synthetase
Article Abstract:
A nonribosomal multienzyme complex is involved in the synthesis of saframycin Mx1. Gene disruption and transposon mutagenesis in Myxococcus xanthus reveal a DNA fragment that is responsible for the synthesis, and sequence analysis indicate that it resembles a peptide synthetase. Saframycins belong to the group of heterocyclic quinone antibiotics such as mitomycins and streptonigrin that can bind DNA and RNA and prevent their synthesis in cells.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1995
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Genetic and functional analysis of genes required for the post-modification of the polyketide antibiotic TA of Myxococcus xanthus
Article Abstract:
Research was conducted to identify genes that are involved in the post-modification steps of TA antibiotic biosynthesis. Results from specific gene disruption analysis indicate involvement of five genes in the post-modification of TA.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1999
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