A novel positive selection for identifying cold-sensitive myosin II mutants in dictyostelium
Article Abstract:
Novel positive selection for myosin heavy chain mutants are generated in soil amoeba Dictyostelium. The selection assumed substrate release of wild-type cells and adherence of myosin null cells after minimal azide exposure. Mutants were localized to a domain encoded by the myosin gene. Some mutant sequences exhibited retention of cold-sensitive properties when expressed in cells without wild-type myosin.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1995
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Progeny of germ line knockouts of ASI2, a gene encoding a putative signal transduction receptor in Tetrahymena thermophila, fail to make the transition from sexual reproduction to vegetative growth
Article Abstract:
Analysis of role of cache domain as signal transduction receptor during replication of deoxyribonuceic acid in Tetrahymena thermophila is presented.
Publication Name: Developmental Biology
Subject: Biological sciences
ISSN: 0012-1606
Year: 2006
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