Use of colistin-polymyxin B-cellobiose agar for isolation of Vibrio vulnificus from the environment

Article Abstract:

Cellobiose-polymyxin B- colistin (CPC) agar was evaluated as compared to other agar media in its efficiency in isolating the human pathogen Vibrio vulnificus which is commonly found in mollusks. Results of the gene probe and monoclonal antibody analyses showed that CPC agar was more selective in isolating Vibrio vulnificus than theother tested media. The use of this medium together with the hemolysin gene probe or anti-flagellar core monoclonal antibody may prove to be very useful inidentifying the pathogen from the environment.

Identification and classification, Microbiology, Cultures (Biology), Microorganisms

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Entry into, and resuscitation from, the viable but nonculturable state by Vibro vulnificus in an estuarine environment

Article Abstract:

The vibrio vulnificus is an estuarine bacterium which can bring about life-threatening infections in people. The emigration of the Vibro vulnificus cells to the viable but nonculturable (VBNC) state explains the inability to obtain the cells from cold estuarine environment. Resuscitation of the cells from the VBNC state in natural conditions leads to a temperature upshift. At low water temperatures the cells enter the VBNC state, but with the rise in temperature the culturable state of the cells is revived.

Analysis, Observations, Resuscitation, Estuaries

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Refined medium for direct isolation of Vibrio vulnificus from Oyster tissue and seawater

Article Abstract:

A new medium which was developed for the direct isolation of Vibrio vulnificus from water and oyster samples is presented.

Science & research, Environmental aspects, Sea-water, Seawater, Oyster-culture, Oyster culture

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