Use of hydrogen peroxide treatment and crystal violet agar plates for selective recovery of bacteriophages from natural environments
Article Abstract:
The use of hydrogen peroxide and crytal violet agar plates led to better recovery of bacteriophages from natural samples. Hydrogen peroxide selectively inactivates indigenous bacteria permitting better phage visualization and recovery. In cases of hydrogen-resistance, bacteria can be eliminated with the addition of crystal violet to the bottom agar used for cell culture. Utilization of hydrogen peroxide and crystal violet allows greater recovery of bacteriophages from natural samples compared to previous methods.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Characterization of a generalized transducing phage of ply-gamma-glutamic acid-producing Bacillus subtilis and its application for analysis of Tn917-LTV1 insertional mutants defective in poly-gamma-glutamic acid production
Article Abstract:
The generalized transducing phage phiBN100 was proposed for the identification and examination of Tn917-LTV1 transpositional mutants that are poly-gamma-glutamic acid production deficient. The phage was derived from a poly-gamma-glutamic acid producing Bacillus subtilis strain. The insertional mutants were successfully detected through the 42kb genome size of the phiBN100.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Method for host-independent detection of generalized transducing bacteriophages in natural habitats
Article Abstract:
A method is described for identifying bacteria that serve as hosts for bacteriophages when the host is unknown. Phage DNA is used as a template for PCR amplification of 16S ribosomal DNA using 16S rRNA genes of eubacteria.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
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