Molecular characterization of the toxic cyanobacterium Cylindrospermopsis raciborskii and design of a species-specific PCR

Article Abstract:

PCR analysis shows that both morphotypes of Cylindrospermopsis raciborskii, straight or coiled trichomes, belong to the same species. PCR was used to amplify a 609-bp region of rpoC1, a DNA-dependent RNA polymerase gene.

Author: Schembri, Mark A., Wilson, Kim M., Baker, Peter D., Saint, Christopher P.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000

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Varied diazotrophies, morphologies, and toxicities of genetically similar isolates of Cylindrospermopsis raciborskii (Nostocales, Cyanophyceae) from Northern Australia

Article Abstract:

The use of different nitrogen sources can change the morphology of the cyanobacterium Cylindrospermopsis raciborskii. This could make it difficult to identify the bacterium, since morphological differences are used taxonomically. Analysis of the HIP1 octameric-palindrome repeated sequence may be a better choice.

Author: Neilan, Brett A., Saker, Martin L.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001

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Cell culture-taqman PCR assay for evaluation of Cryptosporidium parvum disinfection

Article Abstract:

Research has been conducted on Cryptosporidium parvum. The authors report the use of a cell culture-quantitative PCR assay developed for the evaluation of C. parvum inactivation with disinfectants, and demonstrate that the assay is reliable and sensitive.

Author: Saint, Christopher P., Keegan, Alexandra R., Fanok, Stella, Monis, Paul T.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
Australia, Science & research, Pharmaceutical Preparation Manufacturing, Pharmaceutical preparations, Antiinfective Preparations, Analysis, Physiological aspects, Anti-infective agents, Environmental aspects, Microbiology, Coccidia, Cryptosporidium, Microbial ecology, Cell culture

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Subjects list: Usage, Genetic aspects, Bacteria, Cyanobacteria, Polymerase chain reaction, Morphology (Biology), Research
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