Which transcription factor assay should you use?
Article Abstract:
DNA sequences can be matched with their cognate binding proteins by 5 different pathways or assays. Protein arrays, microsphere arrays, chromatin immunoprecipitation, oligonucleotide arrays and ELISA (enzyme-linked immunosorbency assay)-based assays are the different types of signaling pathway methodologies to identify proteins responsible for regulating transcriptional changes.
Publication Name: The Scientist
Subject: Biological sciences
ISSN: 0890-3670
Year: 2006
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Five questions on QPCR
Article Abstract:
Experts have offered solutions from picking standards to finding those low-copy transcripts for the real-time quantitative PCR (QPCR) users. Primer and probe design, sample preparation and normalization control selection are some of the common problems faced by the QPCR users.
Publication Name: The Scientist
Subject: Biological sciences
ISSN: 0890-3670
Year: 2006
User Contributions:
Comment about this article or add new information about this topic:
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