Xylitol formation and reduction equivalent generation during anaerobic xylose conversion with glucose as cosubstrate in recombinant Saccharomyces cerevisiae expressing the xyl1 gene
Article Abstract:
Saccharomyces cerevisiae cells containing the xyl1 gene are able to convert xylose into xylitol effectively only if glucose is present. If the concentration of glucose is high a large amount of xylitol and ethanol is formed and the excess reduction equivalents are used in the dehydrogenation of ethanol. However, if the concentrations of glucose is low the reduction equivalents are used in the oxidation of ethanol. Xylitol formation decreases if the concentrations of xylose in the cell decreases and ethanol and acetate accumulate in the cells.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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Influence of cosubstrate concentration on xylose conversion by recombinant, XYL1-expressing Saccharomyces cerevisiae: a comparison of different sugars and ethanol as cosubstrates
Article Abstract:
The characteristics of xylose conversion to xylitol by recombinant, XYL1-expressing Saccharomyces cerevisiae were analyzed in the presence of maltose, galactose and ethanol cosubstrates. Xylose conversion by recombinant Saccharomyces cerevisiae in anaerobic batch fermentations with glucose, mannose, maltose and galactose indicated the role of reduced cofactors in regulating the synthesis of xylitol. Furthermore, elevated concentrations of the cosubstrates inhibited the conversion of xylose.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Ethanolic fermentation of xylose with Saccharomyces cerevisiae harboring the Thermus thermophilus xylA gene, which expresses an active xylose (glucose) isomerase
Article Abstract:
A functional metabolic pathway which results in the formation of ethanol during oxygen-limited xylose fermentation has been identified in a strain of Saccharomyces cerevisiae. This strain expresses the Thermus thermophilus xylA gene that encodes xylose isomerase. The recombinant xylose isomerase exhibits greatest activity at a temperature of 85 degree celsius with a specific activity of 1.0 U/mg. Xylitol and acetic acid are formed as the by-products of this fermentation.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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