Zinc- and arsenic-resistant strains of Thiobacillus ferrooxidans have increased copy numbers of chromosomal resistance genes

Article Abstract:

Thiobacillus ferrooxidans is capable of metabolizing toxic metals such as zinc and arsenic apart from iron and sulfur. This capability is not concentrated in one strain, but the resistance ability can be introduced by extracting DNA fragments from metal-resistant strains. The endonuclease Xbal was most successful in isolating the resistance genes of T. ferrooxidans, and restriction fragment analysis points to the high availability of these genes in resistance strains.

Gram-negative bacteria

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Phenol hydroxylase cloned from Ralstonia eutropha strain E2 exhibits novel kinetic properties

Article Abstract:

A study was conducted to examine the kinetic characteristics of a phenol hydroxylase cloned from the phenol-degrading bacterium Ralstonia eutropha strain E2. Results indicated that the cloned phenol hydroxylase is a multicomponent enzyme. The kinetic constants of the phenol-oxygenating activity indicated that the enzyme affects K(sub S) and K(sub SI) values for the cellular phenol-oxygenating activity.

Phenols, Phenols (Class of compounds), Microbial enzymes, Enzyme kinetics

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Molecular characterization of a deletion/duplication rearrangement in tfd genes from Ralstonia eutropha JMP134(pJP4) that improves growth on 3-chlorobenzoic acid but abolishes growth on 2,4-dichlorophenoxyacetic acid

Article Abstract:

Results demonstrate the metabolic plasticity of Ralstonia eutropha JMP134 in that the bacterium is able to grow in the presence of 3-chlorobenzoate or 2,4-dichlorophenoxyacetone by enzymaticlly degrading the pollutants. Data further show that the bacterium loses the ability to grow on 2,4-D when cultured in the presence of 3-CB.

Germany, Chile, Physiological aspects, Pollutants, Structure-activity relationships (Biochemistry), Chromosome deletion, Antibody diversity

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