Differential quantitative analysis of MHC ligands by mass spectrometry using stable isotope labeling
Article Abstract:
Different methods based on mass spectrometry consisting of stable isotope labeling are used for determining the differential quantitative analysis of MHC ligands. The first method, which is fast and simple uses acetylation and the second method consisting of a combination of guanidination and nicotinylation, provides higher peptide recoveries and finer sensitivities, are used for a pairwise quantitative comparison for the identification of overpresented MHC ligands.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2004
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Protein chip fabrication by capture of nascent polypeptides
Article Abstract:
Two similar strategies are reported to fabricate protein chips through capture onto a solid surface of the nascent polypeptides during translation of synthetic or in vitro-transcribed RNAs. Study claimed that when a ribosome encounters a double-stranded RNA or RNA-DNA hybrid region, it stalls on the microRNA molecule without being released immediately.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2006
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Quantitative mouse brain proteomics using culture-derived isotope tags as internal standards
Article Abstract:
A study is carried out on an approach based on the use of culture-derived isotope tags (CDITs) for quantitative tissue proteome analysis. This study has identified and quantified a total of 1,000 proteins, 97-98 % of which were expressed in both mouse whole brain and Neuro2A cells.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2005
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