Sequencing genomes from single cells by polymerase cloning
Article Abstract:
A sequencing technique using real-time isothermal amplification to form polymerase clones (plones) from the DNA of single cells and remove the culturing of microorganisms is proposed which provide a critical tools for synthetic characterization of genome sequencing standards. The two Escherichia coli plones on analyzing by Affymetrix chip hybridization demonstrate that plonal amplification is specific and the bias is randomly distributed.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2006
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Metagenomic analysis of two enhanced biological phosphorus removal (EBPR) sludge communities
Article Abstract:
A metagenomic analysis of two lab-scale enhanced biological phosphorus removal (EBPR) sludges dominated by the uncultured bacterium, 'Candidatus Accumulibacter phosphatis' is presented. Inspite of an apparent lack of phylogenetic overlap in the flanking communities of the two sludges studied, common functional themes are found, at least one of them complementary to the inferred metabolism of the dominant organism.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2006
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Recombinant expression of selectivity sulfated proteins in Escherichia coli
Article Abstract:
The selective incorporation of sulfotyrosine into proteins in bacteria by genetically encoding the modified amino acid in response to the amber nonsense codon TAG is reported. This strategy enables direct expression in Escherichia coli of sulfo-hirudin, which was previously inaccessible through recombinant methods and facilitates further study and application of tyrosine sulfation.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2006
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