Coupled interactions among solute diffusions, membrane surface potentials, and opposing enzyme reactions as a mechanism for active transports performed with biomimetic membranes

Article Abstract:

An analysis demonstrated that the membrane surface potential effect of a uniformly charged artificial membrane could be substituted by an electrical resistance term, which balances the passive membrane resistance term. Depending on the sign of this electrical resistance term, the global membrane resistance for a charged solute may be either enhanced or lowered.

Biochemistry, Research and Development in the Physical, Engineering, and Life Sciences, Observations, Chemistry, Analytic, Analytical chemistry, Solution (Chemistry), Solutions (Chemistry)

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Conformational cycle of a single working enzyme

Article Abstract:

A diffusional model presented reveals the coupling of conformation change with enzyme action by simultaneous analysis of the on-time distribution and autocorrelation function of a single working cholesterol oxidase enzyme. Active-site oxidation induces a conformational change that opens the path for substrate entry.

Cholesterol

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Comment on "effect of active site mutation phe93 - Trp in the Horse Liver alcohol dehydrogenase enzyme on catalysis: a molecular dynamics study"

Article Abstract:

The same Boltzmann probability law governs enzyme catalysis as other reactions in condensed phases. Simulations that actually examined the activation of enzyme catalysis indicate that the enzyme reduces the activation barrier by providing a preorganized polar environment that stabilizes the transition rate.

Usage, Molecular dynamics, Erythropoietin, Chemical properties

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