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Diffusion of lipid-like single-molecule fluorophores in the cell membrane

Article Abstract:

The photophysical and diffusive behaviors of the dicyanomethylenedihydrofuran (DCDHF) lipid analogues in membranes are described and are found to be competitive with the well-known lipid probe. It is concluded that several members of a new class of single-molecule emitters could serve as useful lipid analogues in the plasma membrane of Chinese hamster ovary (CHO) cells, with sufficient brightness and stability to allow tracking of single-molecule diffusion.

Science & research, Research, Physiological aspects, Cell membranes, Lipids

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Probing the sequence of conformationally induced polarity changes in the molecular chaperonin GroEL with fluorescence spectroscopy

Article Abstract:

Hydrophobic interactions play a major role in binding non-native substrate proteins in the central cavity of the bacterial chaperonin GroEL. The sequence of local conformational changes by which GroEL and its cofactor GroES assist in protein folding can be explored using the polarity-sensitive fluorescence probe Nile Red.

Methods, Usage, Conformational analysis, Fluorescence spectroscopy, Molecular chaperones

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Bulk and single-molecule characterization of an improved molecular beacon utilizing H-dimer excitonic behavior

Article Abstract:

A pair of fluorophores in the new dicyanomethylenedihydrofuran (DCDHF) dye family was used to design and characterize a new fluorescent probe for nucleic acid detection, referred as a self-quenched intramolecular dimer (SQuID) molecular beacon (MB).

Industrial Organic Chemicals, Cyclic Crude and Intermediate Manufacturing, Furan, Analysis, Fluorescence, Optical properties, Bleaching, Furans, Structure, Methyl groups, Methyl compounds

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