Discrimination between single Escherichia coli cells using time-resolved confocal spectroscopy
Article Abstract:
A technique for rapidly discriminating between single-cell populations within a flowing microfluidic stream is reported. Single-cell time-correlated single-photon counting (scTCSPC) and photon burst spectroscopy are employed to characterize individual Escherichia coli cells expressed within either green, cyano, or yellow fluorescent protein and the calculated burst width characteristics are found to be largely controlled by the fluorescence quantum yield and adsorption cross section of the proteins used.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2007
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The "Le Chatelier's principle'-governed response of actin filaments to osmotic stress
Article Abstract:
The mechanism of the inhibitory effect of F-actin on osmotic stress-induced water flow is elucidated on the basis of nonequilibrium thermodynamics, addressing a novel problem of how osmotic stress changes the chemical potential of the F-actin. F-actin is shown to have an inhibitory effect on the osmotic stress-driven water flow and this is the first report showing that the Le Chatelier's principle applies to the reaction of biopolymers against equilibrium disturbances such as osmotic stress.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2006
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A Lattice-Gas Cellular Automaton to model diffusion in restricted geometries
Article Abstract:
A new and general Lattice-Gas Cellular Automata (LGCA) consisting in a d-dimensional network of hypercubically arranged cells is developed. The results of the application of the model to the specific problem of geometrical restricted long-range diffusion are utilized to interpret the behaviors of particles in tight confinement.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2006
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- Abstracts: Direct observation of odd-even effect in dilute polymeric solutions: A time-resolved fluorescence anisotropy study
- Abstracts: Direct observation of odd-even effect in dilute polymeric solutions: A time-resolved fluorescence anisotropy study. part 2
- Abstracts: Determination of the Fe-CO bond energy in myoglobin using heterodyne-detected transient thermal phase granting spectroscopy
- Abstracts: Primary process of photocarrier generation in Y-form titanyl phthalocyanine studied by electric-field-modulated picosecond time-resolved fluorescence spectroscopy
- Abstracts: Analysis of conformational polymorphism in pharmaceutical solids using solid-state NMR and electronic structure calculations