Metastable liquid clusters in super- and undersaturated protein solutions

Article Abstract:

A Monte Carlo simulation analysis is carried out to exhibit submicron size clusters of dense liquid with protein lumazine synthase as a test system by applying dynamic and static light scattering and atomic force microscopy. The clusters are metastable with respect to the crystals and the low-concentration solution with limited lifetimes and different sizes and it is shown that the average cluster size is decided by the kinetics of growth and decay instead of thermodynamics.

Observations, Optical properties, Bacterial proteins, Atomic force microscopy, Light scattering

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Effects of convective solute and impurity transport in protein crystal growth

Article Abstract:

Research was conducted to examine the effects of convective solute and impurity transport on the crystal growth kinetics of the model protein lysozyme using high-resolution optical interferometry. To quantify changes in unsteady kinetics with flow velocity, Fourier spectra was employed. Results demonstrate that earlier observations of slow protein crystal growth kinetics are related to step bunch formation in response to nonsteady step generation.

Research, Growth, Proteins, Crystals, Protein structure, Lysozyme, High resolution spectroscopy, Crystal growth

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Corresponding-states laws for protein solutions

Article Abstract:

Corresponding-states laws for protein solutions by van der Waal are examined by Monte Carlo simulations with isotropic, pair-additive system. It was found that van der Waals law is best obeyed and appears more general than its original formulation and it was shown that dependencies of the crystal solubility cannot be predicted based on corresponding-states laws.

Usage, Protein research

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