Polarity sensing by fluorescent oligonucleotides: first demonstration of sequence-dependent microenvironmental changes in the DNA major groove
Article Abstract:
The characterization of the major groove of DNA as more polar than the minor groove was demonstrated by using fluorescent oligonucleotides. The experiment, which also showed that the DNA double helix possess varying polarities in its two grooves, is the first demonstration of sequence-dependent microenvironmental effects in DNA in solution. The procedure has potential applications in the study of microenvironmental effects in DNA polymorphs and chemically modified DNA and their hybrids.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 1999
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Physical-chemistry-based analysis of Affymetrix microarray data
Article Abstract:
Publicly available data on Affymetrix microarray spike-in experiments on the human HGU133 chipset in which sequences are added in solution at known concentrations are analyzed. Results suggest that the observed deviations from the predicted collapse are related to the choice of probes or have a biological origin rather than being a problem with the proposed model.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2006
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Studies on the formation of DNA - cationic lipid composite films and DNA hybridization in the composites
Article Abstract:
The formation of composite films of double-stranded DNA and cationic lipid molecules and the hybridization of complementary single-stranded DNA molecules in such composite films are illustrated. This approach shows scope for the synthesis of patterned DNA films and consequent application in disease detection and genome sequencing.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2001
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