Polarity sensing by fluorescent oligonucleotides: first demonstration of sequence-dependent microenvironmental changes in the DNA major groove

Article Abstract:

The characterization of the major groove of DNA as more polar than the minor groove was demonstrated by using fluorescent oligonucleotides. The experiment, which also showed that the DNA double helix possess varying polarities in its two grooves, is the first demonstration of sequence-dependent microenvironmental effects in DNA in solution. The procedure has potential applications in the study of microenvironmental effects in DNA polymorphs and chemically modified DNA and their hybrids.

DNA, Polarity (Biology), Fluorescence spectroscopy

---

Physical-chemistry-based analysis of Affymetrix microarray data

Article Abstract:

Publicly available data on Affymetrix microarray spike-in experiments on the human HGU133 chipset in which sequences are added in solution at known concentrations are analyzed. Results suggest that the observed deviations from the predicted collapse are related to the choice of probes or have a biological origin rather than being a problem with the proposed model.

Genetic research, DNA microarrays, Human genome

---

Studies on the formation of DNA - cationic lipid composite films and DNA hybridization in the composites

Article Abstract:

The formation of composite films of double-stranded DNA and cationic lipid molecules and the hybridization of complementary single-stranded DNA molecules in such composite films are illustrated. This approach shows scope for the synthesis of patterned DNA films and consequent application in disease detection and genome sequencing.

DNA-ligand interactions, DNA binding

Similar abstracts:

• Abstracts: Fluorescence probing of temperature-dependent dynamics and friction in ionic liquid local environments
• Abstracts: Resonant and nonresonant vibrational and electronic excitations in trimethylaluminum adsorbed on GaAs. Photophysics of PTCDA and Me-PTCDI thin films: Effects of growth temperatures
• Abstracts: Theory. Ultrafast phase grating studies of heme proteins: observation of the low-frequency modes directing functionally important protein motions
• Abstracts: Direct visualization of the dynamic hydrolysis process of an L-DPPC monolayer catalyzed by phospholipase D at the air/water interface
• Abstracts: Improvement of hydrothermal stability of mesoporous silica using salts: reinvestigation for time-dependent effects

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.