Resonance Raman and FTIR studies of carbon monoxide-bound cytochrome aa3-600 oxidase of Bacillus subtilis
Article Abstract:
Resonance Raman and FTIR spectroscopy of the fully reduced carbon monoxy derivative of the quinol aa3-600 oxidase from Bacillus subtilis reveal two isotope-dependent vibrational modes at 520 and 575 cm-1. Despite the fact that two different heme-protein conformations affect the iron-his bond strength in the ferrous ligand-free form aa3-600, the carbon monoxide-bound adduct has a single conformation. This conformation presents a His-Fe-CO Cu beta moiety that has the same structure as the alpha form found in the mammalian cytochrome c oxidase.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 1998
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Resonance Raman and Fourier transform infrared detection of azide binding to the binuclear center of cytochrome bo3 oxidase from Escherichia coli
Article Abstract:
A study was conducted to analyze the oxidized azide-bound derivative of the quinol cytochrome bo(sub 3) oxidase from Escherichia coli. Resonance Raman and Fourier transform infrared spectroscopy were utilized to carry out the analysis. The enzyme was solubilized in a buffer solution at ph 7.6. Results indicated quantitative differences in the structure of the heme binuclear pockets upon azide binding.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 1999
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Probing the environment of [Cu.sub.B] in heme-copper oxidases
Article Abstract:
Time-resolved step-scan FTIR and density functional theory are used for probing the structural dynamics of [Cu.sub.B] in heme-copper oxidases at room temperature. The small variations in the v(CO) of [Cu.sub.B] are attributed to protein conformational changes in the vicinity of [Cu.sub.B].
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2007
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