Mechanisms of ribozyme-mediated RNA cleavage

Article Abstract:

Research was conducted to study the mechanisms associated with ribozyme-mediated RNA cleavage. Catalytic RNAs incorporate the need for divalent metal ions and require divalent cations for the formation of their three-dimensional structure. Metal ions participate in RNA catalysis in a number of ways, including coordination, electrophilic catalysis, strain, general acid and base catalysis, and stabilization of an anionic leaving or attacking group. Moreover, the RNA subunit of bacterial ribonuclease P cleaves precursor sequences from the ends of pre-tRNA molecules.

Catalytic RNA

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Oxidative nucleobase modifications leading to strand scission

Article Abstract:

Research was conducted to study chemical events originating on the nucleobase that lead the polymer subject to cleavage in a subsequent step. Electrophile reactions were carried out and were divided into oxidizing alkylating and other electrophilic agents. The alkali-induced strand scission may be interpreted as the occurrence of a chemical modification at one of the nucleobase sites. However, phosphodiester linkages were subjected to alkaline hydrolysis in the case of RNA.

Polymers, Hydrolysis

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Linkers and cleavage strategies in solid-phase organic synthesis and combinatorial chemistry

Article Abstract:

It has been possible to demonstrate the wide range of cleavage conditions available to solid-phase chemists. There is a great deal of literature about linker chemistries, but more work needs to be undertaken in this area.

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