Detection of Chlamydia trachomatis by a ligase chain reaction amplification method
Article Abstract:
The ligase chain reaction (LCR) amplification method appears to provide a new, accurate technique for detecting Chlamydia. Chlamydia infection is a sexually transmitted disease. Current techniques miss cases in populations where few persons are infected or when infection does not produce symptoms. This maintains a reservoir of infected persons who continue to transmit disease. Researchers cultured and performed LCR testing on tissue samples from urethral swabs taken from 501 men with urinary tract symptoms suggestive of Chlamydia infection and cervical swabs taken from 167 of their female partners. Overall, 52 patients tested positive by both methods. An additional 17 patients had positive LCR tests and negative cultures. The 17 equivocal samples underwent two additional different tests of which 15 tested positive on one or both tests and were considered positive. Thus, the LCR test accurately identified more cases than cell culture.
Publication Name: Sexually Transmitted Diseases
Subject: Health
ISSN: 0148-5717
Year: 1996
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Patient-administered tampon-collected genital cells in the assessment of Chlamydia trachomatis infection using polymerase chain reaction
Article Abstract:
A new sample collection technique and test may improve testing for chlamydia infection. Traditionally, a professional swabs the cervix and the sample is cultured. Transport requires special care. The new technique uses a tampon inserted by the woman, and the polymerase chain reaction (PCR) test, which does not require special handling. In a group of 1,000 women suspected of chlamydial infection, researchers compared the results of swab sample cultures with PCR test results on samples obtained by swab and tampon. The PCR test was as accurate as culturing, and no PCR tampon test failed to obtain enough sample.
Publication Name: Sexually Transmitted Diseases
Subject: Health
ISSN: 0148-5717
Year: 1996
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The Impact on Accuracy and Cost of Ligase Chain Reaction Testing by Pooling Urine Specimens for the Diagnosis of Chlamydia trachomatis Infections
Article Abstract:
Pooling urine specimens for testing with the C. trachomatis LCx system can save money without sacrificing diagnostic accuracy. The C. trachomatis LCx system uses nucleic acid amplification, which is the most accurate method of diagnosing Chlamydia trachomatis infections.
Publication Name: Sexually Transmitted Diseases
Subject: Health
ISSN: 0148-5717
Year: 1999
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