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Zoology and wildlife conservation

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Abstracts » Zoology and wildlife conservation

DNA fingerprinting transforms the art of cell authentication

Article Abstract:

DNA fingerprinting is apt to increase in use as researchers discover its effectiveness as a means of testing the authenticity of cell cultures and cell lines. The need for such testing became clear in the 1970s when an influx of contaminated cultures hampered cell research. DNA fingerprinting employs DNA probes to check cell line variation, cross-contamination and the stability of working and extended cell stocks. The method's simplicity and efficiency make it an excellent complement to the two existing methods, isoenzyme analysis and cytogenetic analysis.

Author: Stacey, Glyn N., Bolton, Bryan J., Doyle, Alan
Publisher: Macmillan Publishing Ltd.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1992
Cell culture, Tissue culture

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Heteroplasmy and the Tsar

Article Abstract:

Study of the exhumed remains of Tsar Nicholas II and his youngest brother, Georgij Romanov, by genomic DNA typing shows that both are heteroplasmic at position 16169, thus confirming that the remains from a grave in Ekaterinburg are those of the Romanov family. Resolution of the mitochondrial sequencing discrepancy shows that the two brothers inherited different proportions of cytosine and thymine from their mother. The mixture of cytosine and thymine at the same position in the DNA sequence leads to heteroplasmy.

Author: Debenham, Paul G.
Publisher: Macmillan Publishing Ltd.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1996
Research, Methods, Chromosome mapping, Nicholas II, Emperor of Russia

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DNA fingerprints of cell lines

Article Abstract:

The use of DNA fingerprinting to identify cell lines suffers from the lack of reference standards for unknown contaminants. Isozyme typing, cytogenetics and immunophenotyping provide a more accurate analysis of cell lines, but such exhaustive characterization is too expensive and time consuming for most culture collections to execute. DNA profiling offers a useful alternative.

Author: Stacey, Glyn N., Bolton, Bryan J., Doyle, Alan, MacLeod, Roderick A.F.
Publisher: Macmillan Publishing Ltd.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1992

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Subjects list: Usage, Testing, DNA testing, DNA identification, Cell lines
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