Infiltration of mariner elements

Article Abstract:

The active mariner transposons in planarians, discovered through the polymerase chain reaction (PCR) procedure, will help in developing the mariner elements as an integrative system for a wide range of animal species or phyla. Different subfamilies of mariner transposon elements found in the same species indicate that the phylogenetic distribution of mariner in insects is caused by the horizontal transfer and an early deviation from stochastic loss in some branches. The wide host distribution in insects and the integration in the Drosophila species show that the mariner element may be used as a vector for the stable transformation in anthropods.

Usage, Polymerase chain reaction, Letter to the Editor

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Transposons hop out of maize

Article Abstract:

Transposons, which exist in many living organisms, consist of mobile DNA pieces whose existence was first noted in a 1948 research on maize. The study by Barbara McClintock found that a transposon which lands on a gene will serve as a mutagen as well as a gene tag. In the first researches, gene tagging was found to be effective in plant species with well-characterized transposons. However, later studies have utilized Activator, a maize transposon, in tobacco plants to demonstrate its effect on mutation and trasfer to new sites in the genome. This has made it possible for scientists to isoalte by segregation, the elements causing mutation.

Genetic aspects, Plant genetics, Corn

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Transposon tagging of a male sterility gene in Arabidopsis

Article Abstract:

The process of isolating a transposoon-tagged male sterile mutant in Arabidopsis thaliana with the use of the Enhancer-Inhibitor derived from maize was studied. A transformation vector consisting of non-mobile Enhancer/Suppressor-mutator, an Inhibtor/defective Suppressor-mutator and a hygromycin phosphotransferase gene. Results showed that production of the male sterility phenotype and consequently, of the gene named Male Sterility 2 can be attributed to an Enhancer-transposase-mediated Inhibitor element.

Genetic markers, Arabidopsis

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