Lambing by nuclear transfer

Article Abstract:

A study on cloning lambs by nuclear tranfer from artificially-grown cells was able to conceive the first ever reported live mammalian offspring by the said method. The effectivity rate of the process was found to be low. It was recommended in this light to hasten the development of cultured cells from the blastocyst stage. This is done with the use of an appropriate functional reprogramming of the donor nucleus and a compatible recipient cytoplasm.The two deciding factors to the effectivity of nuclear transfer were cited as recipient ovulated oocytes and the cell cycle age of the donor nuclei.

Methods, Laboratory animals, Sheep, Embryology, Animal embryology, Sheep as laboratory animals

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Dolly is a clone - and no longer alone: After the furore that surrounded the arrival of Dolly, the first mammal to be cloned from differentiated adult cells, doubts were raised that se really was a clone

Article Abstract:

Ashworth and colleagues and Signer at colleagues, have now shown that Dolly, is the direct descendant of an udder cell derived from a Finn Dorset ewe, after doubts were raised that she really was clone. Their experiments compared DNA isolated from frozen udder tissue, cell culture derived from it and Dolly's blood, finding that the three samples were identical. The technique has been further validated by the cloning of mice reported by Wakayama and colleagues.

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First cleavage plane of the mouse egg is not predetermined but defined by the topology of the two apposing pronuclei

Article Abstract:

The studies of experimentally manipulated embryos have led to the conclusion that the polarity of the mouse embryo remains undetermined until the blastocyst stage. The pronuclear transfer experiments showed that the first cleavage plane is not determined in the early interphase but is specified by the newly formed topology of the two pronuclei.

Science & research, Animal embryo, Reproductive technology, Zygote intrafallopian transfer

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