Production of the antimalarial drug precursor artemisinic acid in engineered yeast

Article Abstract:

A study of the engineering of Saccharomyces cerevisiae to produce high titres of artemisinic acid using an engineered mevalonate pathway, amorphadiene synthase, and a novel cytochrome P450 monooxygenase from A. annua that performs a three-step oxidation of amorpha-4, 11-diene to artemisinic acid is presented. The synthesized artemisinic acid is transported out and retained on the outside of the engineered yeast, meaning that a simple and inexpensive purification process can be used to obtain the desired product.

Author: Kirby, James, Keasling, Jay D., Newman, Karyn L., Ro, Dae-Kyun, Paradise, Eric M., Ouellet, Mario, Fisher, Karl J., Ndungu, John M., Ho, Kimberly A., Eachus, Rachel A., Ham, Timothy S., Chang, Michelle C.Y., Withers, Sydnor T., Shiba, Yoichiro, Sarpong, Richmond
Publisher: Macmillan Publishing Ltd.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 2006
Product development, Pharmaceutical Preparation Manufacturing, Antimalarial & Antifungal Preps, Drug therapy, Malaria, Antimalarials

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Amplification of histone genes by circular chromosome formation in Saccharomyces cerevisiae

Article Abstract:

A study shows that a different mechanism of dosage compensation, at the level of gene copy number, can occur when HTA1-HTB1(the histones H2A and H2B are encoded by two gene pairs in Saccharomyces cerevisiae) is deleted and HTA2-HTB2 amplifies via creation of a new, small, circular chromosome. The results have demonstrated another mechanism by which histone gene dosage is controlled to maintain genomic integrity.

Author: Winston, Fred, Libuda, Diana E.
Publisher: Macmillan Publishing Ltd.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 2006
Genetic aspects, Histones, Genetic code

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Golgi maturation visualized in living yeast

Article Abstract:

A direct test of the two models cisternal maturation model and stable compartment model is carried out using three-dimension time-lapse fluorescence microscopy of the yeast Saccharomyces cerevisiae. The results of the test reveal that individual cisternae mature, and do so at a consistent rate.

Author: Glick, Benjamin S., Losev, Eugene, Reinke, Catherine A., Jellen, Jennifer, Strongin, Daniel E., Bevis, Brooke J.
Publisher: Macmillan Publishing Ltd.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 2006
Golgi apparatus

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Subjects list: Research, United States, Brewer's yeast, Saccharomyces cerevisiae
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