Protein images obtained by STM, AFM and TEM

Article Abstract:

Scanning tunneling microscopy (STM) and atomic force microscopy (AFM), two new methods for examining proteins and other biomolecular structures, should be used with established techniques such as transmission electron microscopy (TEM). The three methods were evaluated by comparing their images of the same protein molecule, turtle alpha-macroglobulin. STM and AFM have several advantages such as the ability to examine proteins under physiological conditions. However, TEM was better at delineating the boundaries of molecules. ATM, STM and TEM should complement rather than exclude each other.

Evaluation, Transmission electron microscopes, Scanning tunneling microscopy, Atomic force microscopy

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Beyond silver staining

Article Abstract:

Radioactivation of silver-treated protein molecules is a novel method of purification that will allow biochemists to isolate new proteins in subpicogram amounts. The existing methods that rely on Coomassie blue and silver staining have the disadvantage of needing large quantities of protein material obtained through separation on sodium dodecyl sulphate-polyacrylamide gels. The new method refines the use of silver staining by combining it with a modified sulphur toning procedure involving the radioisotope 35S thiourea; this allows the quantity of silver used to be reduced three fold.

Identification and classification, Proteins, Radioactivation analysis, Activation analysis

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More than transparent

Article Abstract:

Ebbesen and colleagues have reported that metal grids may not be as impervious to radiation as had previously been thought. They found that silver, which is a good conductor of electricity, in film form deposited on quartz, was extremely transparent, and that there was a strong transmission of radiation with wavelengths larger than the hole diameter. This appears to be the first observation of selective transmission, and the explanation may rest with the excitation of surface plasmons.

Research, Radiation, Radiation (Physics), Observations, Photonics

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