Sequential activation of ICE-like and CPP32-like proteases during fas-mediated apoptosis
Article Abstract:
Fas ligand sequentially stimulates CPP32- and interleukin-1-beta-converting enzyme (ICE)-like proteases. CPP32 along with other elements in the cytoplasm are responsible for apoptosis of nuclei. Inhibitors of ICE- and CPP32-like proteases abolish Fas-regulated apoptosis. The cytosol exhibits ICE-dependent and CPP32-like activity, while the cytosolic fraction of Fas-activated cells has transient ICE-like activity. Mouse lymphoma cell lysates with ICE and CPP32 cause apoptosis of nuclei.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
A caspase-activated DNase that degrades DNA durig apoptosis, and its inhibitor ICAD
Article Abstract:
Animal homeostasis is controlled by cell growth and differentiation, as well as cell death, or apoptosis. Apoptosis leads to degradation of chromosomal DNA. Experiments have led to the identification of a caspase-activated deoxyribonuclease (CAD) and its inhibitor (ICAD) in the cytoplasmic fraction of mouse lymphoma cells. It is believed that ICAD acts as a chaperone for CAD during synthesis, remaining mixed with CAD to restrict DNase activity.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis
Article Abstract:
A caspase-activated deoxyribonuclease (CAD) and its inhibitor (ICAD) has been identified and molecularly cloned. It is shown that capase 3 cleaves ICAD, inactivating its CAD-inhibitory effect. Human Jurkat cells were transformed with ICAD-expressing plasmid, but did not lead to DNA degradation. ICAD transformants also resisted stauro-sporine-induced DNA degradation.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Tyrosine kinases in activation of the MAP kinase cascade by G-protein-coupled receptors. Direct stimulation of Bruton's tyrosine kinase by Gq-protein alpha-subunit
- Abstracts: Orientation of Polyergus rufescens (Hymenoptera, Formicidae) during slave-making raids. Individual and social components of wood ant response to conifer sawfly defence (Hymenoptera: Formicidae, Diprionidae)
- Abstracts: Structural basis for selective inhibition of cyclooxygenase-2 by anti-inflammatory agents. Unique fold and active site in cytomegalovirus protease
- Abstracts: Survival of spectacled eider adult females and ducklings during brood rearing. Estimating prefledging survival: allowing for brood mixing and dependence among brood mates
- Abstracts: Statistical associations between restriction fragment length polymorphisms and quantitative traits in beef cattle