Sheep cloned by nuclear transfer from a cultured cell line
Article Abstract:
Lambs were produced using nuclear transfer from epithelial cell lines derived from sheep embryos (TNT4). These TNT4 were catalyzed to quiesce by serum deprivation before their nuclei were fused with enucleated oocytes. The resulting embryos were deposited to temporary ewes and then monitored for seven days afterwhich morulae and blastocysts were transferred to blackfaced ewes and allowed to mature. The development of reconstructed embryos by nuclear transfer was observed to rely on the suitability of the donor nucleus and the recipient cytoplasm.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1996
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Viable offspring derived from fetal and adult mammalian cells
Article Abstract:
It is proven that lambs can be created by transplanting nuclei from cell lines established even from older embryos. Cells obtained from a day-9 embryo, a day-26 fetus and the mammary gland of a six-year-old ewe and transferred to enucleated unfertilized eggs resulted in the birth of live lambs. This indicates that differentiation of the adult cell does not involve irreversible changes in genetic material for normal development to term.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1997
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Production of gene-targeted sheep by nuclear transfer from cultured somatic cells
Article Abstract:
Research is presented concerning the production of living sheep by nuclear transfer following the use of reproducible and efficient targeting of the ovine alpha 1 procollagen locus and the placement of therapeutic transgenes.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 2000
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