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Zoology and wildlife conservation

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Abstracts » Zoology and wildlife conservation

Spliced leader RNAs from lower eukaryotes are trans-spliced in mammalian cells

Article Abstract:

The mechanism by which mammalian cells splice RNA can effect trans-splicing using spliced leader (SL) RNAs. This involves the interaction of a 5' splice site in the SL RNA and a 3' splice site in pre-messenger RNAs. This is established for both in vitro and in vivo mammalian cells. The similarity of of trans-splicing ability by both nematode and mammalian RNA at the 3' sites reveals that the splicing machinery has been conserved between mammals and lower eukaryotes.

Author: Maniatis, Tom, Bruzik, James P.
Publisher: Macmillan Publishing Ltd.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1992

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Local domains of supercoiling activate a eukaryotic promoter in vivo

Article Abstract:

Linear DNA transcription templates are much less effective than circular templates in Xenopus oocytes. However, circular Xenopus ribosomal RNA transcription templates may be replaced partially by transcription by phage T7 RNA polymerase. This suggests that supercoiled transient domains can fulfill the topological needs of Xenopus rRNA transcription.

Author: Dunaway, Marietta, Ostrander, Elaine A.
Publisher: Macmillan Publishing Ltd.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1993
DNA, Genetic transcription, Transcription (Genetics)

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The case for an RNA enzyme

Article Abstract:

Issues concerning new evidence on RNA splicing is discussed. Splicing is the reaction that takes place which enables the messenger RNA to become functional.

Author: Nilsen, Timothy W.
Publisher: Macmillan Publishing Ltd.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 2000

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Subjects list: Research, RNA splicing
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