A 10.3 kbp segment from nprB to argJ at the 102-degree region of the Bacillus subtilis chromosome

Article Abstract:

Chromosome walking strategy using the integrative plasmid pDIA5304 and subsequent restriction enzyme analysis were done to locate and isolate the 10336-bp region from nprB to argJ of the Bacillus subtilis chromosome. The cloned gene was identified and selected by Southern hybridization. DNA sequencing using commercially available kits revealed that within the region, there are 12 open-reading frames, four known genes, namely argJ, argC, ipi and nprB and two other genes yitY and yitS. The products of the latter two were found to display homology with L-gulono-gamma-lactone oxidase of rat and dihydrofolate reductase of Staphylococcus aureus.

Usage, Nucleic acid hybridization, Cloning, Chromosome mapping, DNA sequencers, Restriction enzymes, DNA, DNA restriction enzymes

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Sequencing of regions downstream of addA (98 degrees) and citG (289 degrees) in Bacillus subtilis

Article Abstract:

A research on the chromosomal region between addA and agrJ was conducted to determine the nucleotide sequencing on the Bacillus subtilis. Putative ORFs were identified through using the DNA sequencer. The findings of the study revealed that three putative ORFs of 17.3 kbp sequence of addA (98 degrees) corresponded with addA, sbcD and wprA genes of B. subtilis. Furthermore, among the 10 putative ORFs identified in the sequencing of 7.6 citG (189 degrees), two also corresponded with the citG and mrgA genes of B. subtilis.

Nucleotide sequence, Base sequence, Chromosomes

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A Bacillus subtilis chromosome segment at the 100 degrees to 102 degrees position encoding 11 membrane proteins

Article Abstract:

A research on the 25.9 kbp region of nprB between 100 degrees to 102 degrees on the Bacillus subtilis chromosome was conducted to identify the putative ORFs found within it. The Blast program was the primary methodology used in the sequencing. Findings included the analysis that yisZ, yitA and yitB indicated similarities with sulphate adenylyl metabolism enzymes. Two proteins were also found within yisY esterase. Furthermore, 11 ORFs with several membrane domains were found in the study.

Membrane proteins

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