A DNA microarray platform based on direct detection of rRNA for characterization of freshwater sediment-related prokaryotic communities

Article Abstract:

A development and evaluation of a DNA microarray platform for the characterization of bacterial communities is presented in freshwater sediments based on a heterogeneous set of 70 16S rRNA-targeted oligonucleotide probes and directly labeled environmental RNA. Hybridization from environmental samples were validated by parallel fluorescence in situ hybridization combined with catalyzed reported deposition for maximizing sensitivity of the analysis.

Genetic aspects, Prokaryotes, RNA processing

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Improved in situ hybridization efficiency with locked-nucleic-acid-incorporated DNA probes

Article Abstract:

A study demonstrates remarkable improvement in rRNA-targeted in situ hybridization efficiency by applying locked-nucleic-acid (LNA)-incorporated oligodeoxynucleotide probes (LNA/DNA probes) without compromising specificity. The findings of the study suggest that the introduction of LNA residues in DNA will prove to be a useful approach for effectively increasing probe hybridization efficiency.

Japan, Biological Product (except Diagnostic) Manufacturing, Drugs, DNA Probes, Structure

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Diversity of anaerobic microorganisms involved in long-chain fatty acid degradation in methanogenic sludges as revealed by RNA-based stable isotope probing

Article Abstract:

RNA-based stable isotope probing was used for examining long-chain fatty acid (LCFA)-degrading microbes. Findings reveal the active in situ participation of phylogenetically diverse bacterial groups in the degradation of LCFA under methanogenic conditions.

Biodegradation, Biological diversity, Biodiversity, Phylogeny

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