A linkage map of sweet cherry based on RAPD analysis of a microspore-derived callus culture population

Article Abstract:

A microspore-derived callus culture population is used to construct a fractional linkage map for the sweet cherry, Prunus avium L. cultivar Emperor Francis. Seventy-nine random decanucleotide DNA primers and the polymerase chain reaction are used to genotype two allozymes and 90 random amplified polymorphic DNA (RAPD) markers. DNA blot and hybridization using five cloned RAPDs indicate that one of the decanucleotide primers amplifies a region of the Emperor Francis genome having a rare sequence and the other four amplify regions with repeated sequences.

Analysis, Observations, Nucleic acid hybridization, Genetic markers, Chromosome mapping, Cherry, Cherries

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Allozyme variation and genetics in asparagus

Article Abstract:

Genetic markers were developed for the Asparagus officinalis using isozyme methodology. It was shown that among the 34 enzymes studied for potential polymorphism, 13 had adequate activity and resolution in gels for interpretation. Aconitase, endopeptidase, malate dehydrogenase, phosphoglucomutase and shikimate dehydrogenase were found to be polymorphic and can be used as markers in asparagus biotechnology and mapping.

Asparagus, Isoenzymes

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Chloroplast inheritance and DNA variation in sweet, sour, and ground cherry

Article Abstract:

The maternal species origins of sour cherry were investigated using cpDNA markers to screen a wide range of sour, sweet, and ground cherries. Ground cherry was more likely than sweet cherry to be the maternal progenitor species of sour cherry. Two sour cherry selections had prevalent sweet cherry haplotype and a wild French sweet cherry selection had the most prevalent ground cherry haplotype.

Sour cherry, Sour cherries

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