A novel real-time PCR for Listeria monocytogenes that monitors analytical performance via an internal amplification control

Article Abstract:

The development and optimization of a novel quantitative real-time (Q)-PCR assay for Listeria monocytogenes is reported based on the simultaneous detection of hly gene target sequences, which provides high specificity, sensitivity and quantifiability, and an internal amplification control (IAC) sequence for the assessment of PCR inhibition. This Q-PCR assay for Listeria monocytogenes is a simple and robust tool that facilitates the identification of false negatives or underestimations of contamination loads due to PCR failure.

Biological Product (except Diagnostic) Manufacturing, Drugs, Deoxyribonucleic Acid, DNA, Polymerase chain reaction, Listeria monocytogenes

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Gateway-based destination vectors for functional analyses of bacterial ORFeomes: Application to the min system in Brucella abortus

Article Abstract:

The construction of twenty gateway-compatible destination vectors comprising of fluorescent and epitope fusion tags, various drug markers, and replication origins which could be useful for exploring existing microbial ORFeomes is reported. The vectors are observed to be useful to determine the subcellular distribution of several proteins involved in cell division and differentiation of a given bacterium.

Physiological aspects, Gene expression, Cell division, Genetic vectors, Brucella

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